Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 8 pack.
2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 8 pack.
Teknova 0.9% Saline Solution. 1000mL, Sterile. Endotoxin Tested. 8 pack.
0.9% Saline Solution. 1000mL, Sterile. Endotoxin Tested. 8 pack.
Teknova 1M HEPES Solution, pH 6.5. 1000 mL, Sterile. 8 pack.
1M HEPES Solution, pH 6.5. 1000 mL, Sterile. 8 pack.
Teknova 1X TBS, Modified. 1000mL, Sterile. (150mM NaCl, 50mM Tris) 8 pack.
1X TBS, Modified. 1000mL, Sterile. (150mM NaCl, 50mM Tris) 8 pack.
Teknova 1X PBS with 0.1% Tween-20. 1000mL, Sterile. 8 pack.
1X PBS with 0.1% Tween-20. 1000mL, Sterile. 8 pack.
Teknova 10X HBS-P Buffer, pH 7.4 at 1X. 1000mL, Sterile 8 pack.
10X HBS-P Buffer, pH 7.4 at 1X. 1000mL, Sterile 8 pack.
Teknova 10X TBS with Tween-20 Kit, 1000mL, Sterile. (1M Tris, 1.5M NaCl, 0.5% Tween) 8 pack.
10X TBS with Tween-20 Kit, 1000mL, Sterile. (1M Tris, 1.5M NaCl, 0.5% Tween) 8 pack.
Teknova 1M Tris-HCl, pH 8.0. 1000mL, Sterile, Endotoxin Tested, PCR Grade. 8 pack.
1M Tris-HCl, pH 8.0. 1000mL, Sterile, Endotoxin Tested, PCR Grade. 8 pack.